Table 1.
Oligonucleotide sequences
| Designation | Sequence (5′ to 3′) |
|---|---|
| Degenerate | |
| As1 | TTTTTYRTTATCAAWTGWCAATGTGWAAC* |
| As2 | GCYCTYAAYAAATCATARTRRAAGTT |
| MSP1-R1 | GAAGCTGGARGARCTRCAGAA |
| MSP1-R2 | GAAGAACTRCAGAAAAYWCCMTC |
| Plasmodium malariae | |
| PmMSP1-F1 | AATGAAGATTATGAACAACTYATTCAA* |
| PmMSP1-F2 | AAGTTGGGAAAACTGGAGGAAC† |
| PmMSP1-R2 | GAAGGCTCTTTATCTTCAACAC |
| PmMSP1-R3 | CAACACATCCTCCATCAMCTAG |
| Plasmodium ovale | |
| PoMSP1-F1 | CAAGTGGTTAGCAAGTTAACAG |
| PoMSP1-F2 | CGGACATAAGATTCCTTCTATC |
| PoMSP1-R1 | TTGGTACACATGTTTCTCCAAC |
| PoMSP1-R2 | ATCTCCATTCTTCTCGTCCATC |
*
As1 and PmMSP1-F1 (aka Mmsp1) sequences are slightly different than the published primer sequences.8
†
PmMSP1-F2 was designed based on GenBank sequence AF138881.1.