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. 2010 Apr 21;3(3):594–602. doi: 10.1093/mp/ssq014

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© The Author 2010. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Analysis of DNA Methylation and of the Chromatin Status at the TS–GUS Locus.

(A) Graphic representation of the DNA methylation of TS–GUS in response to UV-B and temperature stress. Ordinates correspond to percent methylation in the entire set of samples analyzed by bisulfite sequencing (n = 20). Differences between samples derived from control (top) and stressed (bottom) plants are indicated as bars (middle). Nucleotide positions are indicated at the x-axes.

(B) Chromatin immunoprecipitation performed with chromatin derived from UV-B, heat-stressed, and non-stressed control plants. Top left: histone H3 occupancy at the TS–GUS locus under ambient (control) and stressed conditions. Shown are values after normalization to the corresponding input fraction. Top right; bottom: histone H3 modifications at the TS–GUS locus under ambient (control) and stressed conditions. Values are normalized to control IPs performed with non-discriminating antibodies against histone H3. Standard deviations are indicated as bars.

(C) Comparison of TS–GUS activity in 14-day-old wild-type (WT, left) and rts1-1 (right) plantlets.