Fig. 2.

Double mutant analysis provides information on functional dependencies between genes. (A) “Double mutant” (DM) plot of Δhac1. Each point represents a gene. X-coordinate represents the reporter level of a strain deleted for that gene in a WT background. Y-coordinate represents the reporter level in a double mutant lacking the same gene and additionally deleted for a second gene (in this case HAC1). The horizontal blue line indicates the reporter level in the Δhac1 single mutant. Circled in red are up-regulators whose reporter induction is HAC1-independent, which are highly enriched for chromatin architecture factors. (B) (Top) Schematic of the lumenal steps of the N-linked glycan synthesis pathway. (Bottom) DM plot for Δdie2/alg10. (C) DM plot depicting genetic interactions between deletion mutants and over-expression (OE) of the ERAD substrate KWS.