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. Author manuscript; available in PMC: 2010 May 27.
Published in final edited form as: Science. 2009 Mar 27;323(5922):1693–1697. doi: 10.1126/science.1167983

Fig. 5.

Fig. 5

YOR164C/GET4 and MDY2/GET5 function in the pathway of tail-anchored protein insertion. (A) DM plot depicting the functional dependencies of MDY2/GET5. (B) In-vitro translocation assay. Sec22p was translated in cytosol from wild-type (WT) or Δmdy2/get5 strains. Error bars represent +/− SEM, N=3 (C) GFP-Sed5p localization defect in Δget3, Δget4 and Δmdy2/get5 strains. The images of at least 20 cells per strain with similar average fluorescence were quantified to determine the distribution of each strain’s total fluorescence across pixels of different intensities. (D) Silver stain of immunoprecipitation of Get3-FLAGp from ER microsomes and cytosol; protein identities were determined by mass spectrometry.