Figure 1.

Comparison of two methods of estimating ASE polymorphism. (A–C) Y-axis, log₂(e_IGA) (IGA transcriptome data), versus x-axis, log₂(e_Pyro) (pyrosequencing). (A,B) comparison of co-culture and hybrid results from the two methods, respectively; (C) comparison between IGA sequencing and pyrosequencing for the value log₂(e_Co) − log₂(e_Hy), which is also log₂(e_trans). To test if the regressions differ from equality, we tested the estimated regression coefficients against the null hypothesis H₀: m = 1; b = 0. All P-values for these hypothesis tests for all regression parameters are not significant (all P-values > 0.05), indicating that IGA and pyrosequencing give very similar results.