Skip to main content
. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: J Invest Dermatol. 2009 Jul 30;130(2):415–424. doi: 10.1038/jid.2009.224

Figure 7.

Figure 7

Knockdown of CTGF reduces expression of type I procollagen in primary adult human dermal fibro-blasts. Fibroblasts were transfected with non-specific siRNA (Ctrl) or CTGF siRNA. Total RNA or protein was prepared 48 hours after transfection. (A) CTGF and 36B4 (internal reference for normalization) mRNA levels were quantified by real-time RT-PCR. Data are means + SEM, N=3, *p<0.05. (B) CTGF and s-actin (internal reference for normalization) protein levels were quantified by Western analyses. Insets show representative Western blots. Data are means + SEM, N=3, *p<0.05. (C) Type I procollagen and 36B4 (internal reference for normalization) mRNA levels were quantified by real-time RT-PCR. Data are means + SEM, N=3, *p<0.05. (D) Type I procollagen and s-actin (internal reference) protein levels were quantified by Western analyses. Insets show representative Western blots. Data are means + SEM, N=3, *p<0.05. (E) Fibroblasts were transfected with type I procollagen α2 promoter (COL1A2) CAT reporter and Lac Z reporter (internal control for normalization), with empty vector (pCDNA3.1) and non-specific siRNA (Ctrl), or CTGF expression vector, or CTGF siRNA. CAT and β-galactosidase activities were determined 48 hours after transfection. Data are means + SEM, N=3, *p<0.05.