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. 2010 May 6;2010:315108. doi: 10.1155/2010/315108

Figure 1.

Figure 1

aglD-complemented Hfx. volcanii cells regain the ability to properly glycosylate the S-layer glycoprotein. The protein contents of cells of the WR536 background strain (bkgnd), the same strain deleted of aglD (ΔaglD) or the AglD-lacking strain transformed with a plasmid encoding CBD-AglD (ΔaglD/CBD-aglD) were separated by 5% SDS-PAGE and the S-layer glycoprotein was detected by Coomassie stain (CBB) or periodic acid-Schiff (PAS) reagent. In the presence of CBD-AglD, the migration and positive glycostaining of the S-layer glycoprotein are as observed in the background strain.