Figure 2.

Restoration of ER-to-Golgi transport is specific for coexpression of myc-ykt6 and α-synuclein. (A) NRK cells coexpressing myc-β-galactosidase did not exhibit restored transport. (B) Immunoblot of NRK cell lysates using anti-myc antibody. (C) Scatter plot showing every individual cell from the experiment in part A, as a function of VSV-G-GFP expression and transport index. (D) Transport indices of NRK cells electroporated with either α-synuclein A53T or myc-ykt6 alone (not coexpressed) shows that myc-ykt6 does not itself accelerate transport. Error bars represent SEs.