Figure 6.

α-Synuclein A53T overexpression does not significantly affect the expression of ER/Golgi SNAREs. Electroporated NRK cells were allowed to express the α-synuclein A53T construct for 2 d before fixation, permeabilization, and immunofluorescence labeling of α-synuclein (with FITC) and each ER/Golgi SNARE (with PE). Labeled cell suspensions were analyzed by flow cytometry. Dot plots display relationship between α-syn A53T labeling and labeling for syntaxin 5 (A), sec22b (B), rbet1 (C), and membrin (D), where the frequency of cells with similar labeling intensities is indicated by color warmth. (E) Mock-transfected cells in which primary antibodies were omitted. (F) Mock-transfected cells labeled with anti-α-synuclein and anti-syntaxin 5 antibodies.