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. 2010 Jun 1;21(11):1864–1871. doi: 10.1091/mbc.E09-09-0813

Figure 5.

Figure 5.

Mad3 binds to HAT p300, which induces histone H3 acetylation. (A and B) ChIP analysis of histone H3 acetylation in the Id2 promoter. Chromatin prepared from CD74−/− or control B cells was immunoprecipitated with control or anti-acetylated H3 antibodies. Presence of the promoter sequence was then quantified by RT-PCR (A) and quantitative real-time PCR (B). Results presented are representative of two separate experiments. (C and D) Immunoprecipitations: CD74−/− B220+ B cells were lysed. (C) After anti-p300 immunoprecipitation, proteins were separated on SDS-PAGE and transferred onto nitrocellulose. Mad3 was detected by Western blot analysis. (D) After anti-Mad3 immunoprecipitation, proteins were separated on SDS-PAGE and transferred onto nitrocellulose. p300 was detected by Western blot analysis. The results presented are representative of at least three separate experiments.