Figure 7.

Mad3 negatively regulates B cell differentiation. (A–C) Total splenocytes derived from CD74−/− mice were incubated in the presence of 10 nM Mad3 (A) or Mad4 (B) antisense or sense oligonucleotides at 37°C. After 48 h, B cell subpopulations were analyzed for the mature B population by using anti-B220, anti-CD21, and anti-CD24 antibodies. (C) Graph summarizes the results of four independent experiments. (D) Total splenocytes derived from CD74−/− mice were incubated in the presence of 10 nM Mad3 or Mad4 antisense or sense oligonucleotides at 37°C. After 48 h, B cell subpopulations were analyzed for the mature B population by using anti-AA4.1 antibody. Graph summarizes the results of four independent experiments.