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. 2010 Jun 1;21(11):1872–1884. doi: 10.1091/mbc.E09-09-0839

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© 2010 by The American Society for Cell Biology

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Figure 2. — H3.3 preferentially incorporates into chromatin domains marked by histone modifications of active genes. (A) Colocalization analysis of overlap between H3.3-mCherry and immunolabeled modified histones in MSCs. Correlation coefficients (R) of overlaps were determined in individual cells as in Figure 1C, as shown on the graphs (each graph is for a single cell). (B) Average ± SD of correlation coefficients (R values) of H3.3-mCherry and H3 modification immunolabeling overlaps for each H3 modification in MSCs (n = 40–56 cells). R values for modified H3 and either H2B-diHcRed (21–35 cells) or H3.2-mCherry (n = 24–56 cells) overlaps are also shown. Bars, 10 μm.