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. 2010 Jun 1;21(11):1872–1884. doi: 10.1091/mbc.E09-09-0839

Figure 3.

Figure 3.

H3.3-EGFP ChIP-on-chip validation on target promoters. (A) Combinatorial enrichment profiles detected by ChIP-on-chip for H3.3-EGFP, H3K4me3, H3K9me3, and H3K27me3 on the promoters of indicated genes. (B and C) H3.3-EGFP, H3K4me3, H3K9me3, and H3K27me3 profiles on the PPARG2, MYOG, and GAPDH promoters determined by ChIP-on-chip (B) and ChIP-qPCR 9C). (D) EGFP ChIP-on-chip peak detection (FDR ≤ 0.1) in a 1.7-Mb segment of chromosome 1 in sorted H3.3-EGFP-expressing cells (top track) and control EGFP-expressing cells (bottom track). (E) ChIP-qPCR analysis of control EGFP (left) and H3.3-EGFP (right) enrichment on indicated promoters (data from 4 independent ChIPs each analyzed by duplicate qPCRs).