Figure 6.

RanBP3 modulates the Nup98-Crm1 interaction in a biphasic manner. (A) Cells were transfected with EGFP-Nup98 alone or in combination with RanBP3 for 48 h. The cells were then fixed and examined by confocal microscopy. The nuclei were visualized by DAPI staining. Bar, 10 μm. (B) GST-Nup98 (5 μg) was incubated with Crm1 (200 nM), RanQ69L (1 μM), NES (6 μM), and different concentrations of RanBP3 (from left to right: 11, 33, 100, 300, and 900 nM) for 2 h at 4°C and was precipitated using glutathione-Sepharose 4B beads. The bound proteins were analyzed by immunoblotting.