Table 2.
Growth rate and final cell density of different Z. mobilis strains in the absence or presence of different sodium and acetate ions.
| ZM4 | AcR | AcRIM0347 | AcRIM0347 (p42-0347) | ZM4 (p42-0347) | ||
|---|---|---|---|---|---|---|
| Growth rate (hour-1) | RM | 0.42 ± 0.01 | 0.39 ± 0.01 | 0.32 ± 0.003 | 0.33 ± 0.002 | 0.38 ± 0.003 |
| RM (NaCl) | 0.24 ± 0.008 | 0.29 ± 0.005 | 0.21 ± 0.008 | 0.22 ± 0.009 | 0.25 ± 0.008 | |
| RM (NH4OAc) | 0.20 ± 0.008 | 0.19 ± 0.005 | NA | 0.22 ± 0.002 | 0.19 ± 0.007 | |
| RM (Kac) | 0.15 ± 0.004 | 0.12 ± 0.000 | NA | 0.09 ± 0.003 | 0.12 ± 0.006 | |
| RM (NaAc) | NA | 0.29 ± 0.04 | 0.12 ± 0.004 | 0.16 ± 0.002 | 0.27 ± 0.004 | |
| Final Cell Density (OD600 nm) | RM | 0.95 ± 0.006 | 1.01 ± 0.006 | 0.94 ± 0.004 | 0.92 ± 0.002 | 1.02 ± 0.004 |
| RM (NaCl) | 0.73 ± 0.01 | 0.96 ± 0.01 | 0.73 ± 0.03 | 0.72 ± 0.02 | 0.84 ± 0.01 | |
| RM (NH4OAc) | 0.43 ± 0.01 | 0.42 ± 0.006 | NA | 0.32 ± 0.007 | 0.37 ± 0.008 | |
| RM (Kac) | 0.42 ± 0.002 | 0.40 ± 0.000 | NA | 0.28 ± 0.007 | 0.34 ± 0.004 | |
| RM (NaAc) | NA | 0.63 ± 0.02 | 0.25 ± 0.001 | 0.45 ± 0.002 | 0.59 ± 0.002 | |
"NA" indicates that the data are not available due to the lack of growth in that condition. The concentration for all the chemicals (NaCl, NH4OAc, KAc, NaAc) supplemented into the RM is 195 mM. NaCl: sodium chloride, NH4OAc: ammonium acetate, KAc: potassium acetate, NaAc: sodium acetate. Strains included in this study are: ZM4: Zymomonas mobilis ZM4 wild-type; AcR: previously described ZM4 acetate tolerant mutant; ZM4 (p42-0347): ZM4 containing a gateway plasmid p42-0347 to express ZM4 gene ZMO0347; AcRIM0347: AcR insertional mutant of ZMO0347; AcRIM0347 (p42-0347): AcRIM0347 containing gateway plasmid p42-0347. This experiment has been repeated at least three times with similar result. Duplicate biological replicates were used for each condition.