Table 3.
Growth rate and final cell density of different Z. mobilis strains in the absence or presence of different pretreatment inhibitors.
| ZM4 | AcR | AcRIM0347 | AcRIM0347(p42-0347) | ||
|---|---|---|---|---|---|
| Growth rate (hour-1) | RM | 0.48 ± 0.03 | 0.46 ± 0.003 | 0.35 ± 0.004 | 0.32 ± 0.003 |
| HMF | 0.36 ± 0.02 | 0.35 ± 0.01 | 0.19 ± 0.02 | 0.22 ± 0.001 | |
| Furfural | 0.31 ± 0.01 | 0.30 ± 0.005 | 0.19 ± 0.03 | 0.20 ± 0.01 | |
| Vanillin | 0.26 ± 0.001 | 0.26 ± 0.01 | 0.20 ± 0.006 | 0.20 ± 0.003 | |
| Final Cell Density (OD600 nm) | RM | 0.91 ± 0.01 | 0.98 ± 0.006 | 0.95 ± 0.003 | 0.92 ± 0.006 |
| HMF | 0.93 ± 0.003 | 0.96 ± 0.006 | 0.67 ± 0.03 | 0.78 ± 0.02 | |
| Furfural | 0.88 ± 0.006 | 0.89 ± 0.009 | 0.67 ± 0.001 | 0.80 ± 0.02 | |
| Vanillin | 0.69 ± 0.006 | 0.71 ± 0.01 | 0.66 ± 0.01 | 0.70 ± 0.01 | |
The concentration for the inhibitor supplemented into the RM is: HMF: 0.75 g/L, furfural, or vanillin: 1 g/L. Strains included in this study are: ZM4: Zymomonas mobilis ZM4 wild-type; AcR: previously described ZM4 acetate tolerant mutant; AcRIM0347: AcR insertional mutant of ZMO0347; AcRIM0347 (p42-0347): AcRIM0347 containing gateway plasmid p42-0347. This experiment has been repeated at least three times with similar result. Duplicate biological replicates were used for each condition.