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. 2010 May 27;5(5):e10859. doi: 10.1371/journal.pone.0010859

Figure 3. miR-22 targets PTEN through a site at the 3′UTR.

Figure 3

A schematic representation of a reporter gene used to analyze the miR-22 target site of PTEN 3′UTR is shown in the upper panel. The sequences of the wild type and mutated target site are shown. The wild type and mutated reporter plasmids were co-transfected into HEK293T cells together with miR-22 expression plasmid or the parental vector pCDNA3 and RSV-renilla that served as internal control. The activity of each construct without miR-22 was set to 1. The results represent the average +/− SD of four independent transfection experiments in which the bar marked with * indicates p<0.01.