Figure 3. AA-mediated O₂ scavenging results in induction of DevR-regulated promoter activity.

(A) Time dependent depletion of dissolved O₂ (DO) in Dubos medium with AA concentrations of 1 mM (Δ), 2 mM (○), 5 mM (•), 10 mM (□), 10 mM DHA (×), expressed as% of initial value (saturation). (B) Rv1738 promoter activity leads to induction of GFP fluorescence (expressed in RFU/OD) in AA-treated shaking M. tb cultures. Data is shown as mean ± SD (n = 6) for 0 mM (▪), 1 mM (Δ), 2 mM (○), 5 mM (•), 10 mM (□) of AA. Inset in (B) shows promoter activity in AA-treated cultures at 24 h. The first time point showing a statistically significant induction is shown by the red arrow on the red line in (A). The data that was subjected to statistical analysis is shown in a blue shaded rectangle.