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. 2010 May 27;5(5):e10860. doi: 10.1371/journal.pone.0010860

Figure 4. Differential signaling through DevS/DosT by AA and under gradual hypoxia.

Figure 4

Induction of Rv3134c promoter activity (GFP fluorescence in RFU/OD) was assessed in M. tb cultures treated with (A) 10 mM AA, or (B) standing for various intervals of time. Fold induction is the ratio between GFP fluorescence (in RFU/OD) in treated vs. untreated cultures, all in 96-well microplate standing format. (C) Western blot analysis of lysates for expression of HspX and SigA proteins in aerobic M. tb cultures (Aer) and those exposed for 8 h to 10 mM AA (under shaking aerobic conditions) or in 5-day standing (Hyp) cultures. Results are expressed as mean ± SD of 3 independent experiments. Note that the y-axis range is different in (A) and (B) panels.