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. 2010 May 27;5(5):e10860. doi: 10.1371/journal.pone.0010860

Figure 7. AA-triggered bacterial growth arrest and dormant physiology of M. tb in infected THP-1 cells.

Figure 7

(A) Bacteriostasis within untreated and AA-treated THP-1 cells. In case of ‘AA’-treated THP-1, the cells were treated with AA immediately after bacterial infection (i.e. day 0). Statistically significant difference in CFUs recovered from AA-treated vs. untreated THP-1 cells on various days was determined using paired two-way ANOVA with Bonferroni post-test correction (***, p-value <0.001). (B) Dormant physiology of M. tb within AA-treated THP-1 cells. Infected THP-1 cells were either not treated (‘Untreated’) or treated with 2 mM AA (‘AA’) for 7 days post-infection. INH (4 µg/ml final concentration) was added on day 1 to ‘Untreated+INH’ or ‘AA+INH’cultures, and INH treatment continued until day 7. Viable counts were determined on day 7 post-infection (i.e. 6 days post INH-treatment). ‘Untreated’, 100%, total bacteria on day 7; ‘Untreated+INH”, % bacterial recovery with reference to ‘Untreated’; AA, 100%, total bacteria on day 7; ‘AA+INH’, % bacterial recovery with reference to ‘AA’. Mean ± SD of 3 independent experiments is shown.