Table 2. Escherichia coli K-12 strains used in this study.
Strain(s) | Relevant genotype | Reference or source |
CM5407 | polA6 Tn10 | [52] |
FC29 | Δ(lac-proAB)XIII ara thi [F' ΔlacIZ proAB+] | [28] |
FC36 | Δ(lac-proAB)XIII ara thi RifR | [28] |
FC40 | FC36 [F' lacI33ΩlacZ proAB+] | [28] |
GW2100 | umuC122::Tn5 | [66] CGSCa |
MG1655 | wild-type | [67] |
NR9779 | dnaE486 zae::Tn10dCam | [68] |
NR9915 | dnaE915 zae-502::Tn10 | [53] |
PJH305 | FC40 fadAB3165::Tn10Kan lexA3(Ind−) | SMR3490 x P1(SMR868) |
PJH306 | FC40 polA12(TS) fadAB3165::Tn10Kan lexA3(Ind−) | SMR3491 x P1(SMR868) |
PJH354 | SMR4562 polA1 zih35::Tn10 | independent construct of PJH399 |
PJH373 | SMR4562 dinB10 polA1 zih35::Tn10 [F'dinB10] | [51] |
PJH399 | SMR4562 polA1 zih35::Tn10 | [51] |
PJH491 | SMR4562 polA1 zih35::Tn10 polBΔ1::ΩSm-Sp | PJH354 x P1(SMR3661) |
PJH510 | SMR4562 dinB10 polA1 zih35::Tn10 polBΔ1::ΩSm-Sp [F'dinB10] | PJH373 x P1(SMR3661) |
PJH601 | FC40 ΔumuDC::cat dinB10 [F'dinB10] | SMR5830 x P1 SMR3525 |
RM3980 | MG1655 ΔdnaQ903::tet spq-2 | [69] |
RW120 | ΔumuDC::cat | Roger Woodgate |
SH2101 | polBΔ1::ΩSm-Sp | [47] |
SMR540 | FC40 dnaE486 zae::Tn10dCam | FC40 x P1(NR9779) |
SMR868 | FC40 lexA3(Ind−) | [37] |
SMR1547 | FC40 ΔdnaQ903::tet spq-2 zae::Tn10dCam | FC40 x P1(SMR3640) |
SMR3490 | FC40 fadAB3165::Tn10Kan | [43] |
SMR3491 | FC40 polA12(TS) fadAB3165::Tn10Kan | [43] |
SMR3525 | FC40 ΔumuDC::cat | FC40 x RW120 |
SMR3640 | MG1655 ΔdnaQ903::tet spq-2 zae::Tn10dCam | RM3980 x P1(SMR540) |
SMR3661 | FC40 polBΔ1::ΩSm-Sp | FC40 x P1(SH2101) |
SMR4562 | independent construction of FC40 | [37] |
SMR5400 | SMR4562 lexA51(Def) sulA211 psiB::cat | [37] |
SMR5830 | SMR4562 dinB10 [F'dinB10] | [35] |
SMR5889 | SMR4562 ΔdinB50::FRT [F' ΔdinB50::FRT] | [35] |
SMR6263 | MG1655 leu::Tn10 | MG1655 x P1(ZK2146) |
SMR7518 | SMR4562 umuC122::Tn5 | SMR4562 x P1(GW2100) |
SMR7767 | SMR5400 dnaE915 zae-502::Tn10 | SMR5400 x P1(NR9915) |
SMR7768 | SMR5400 zae-502::Tn10 | SMR5400 x P1(NR9915) |
SMR8363 | SMR4562 zae-502::Tn10 | SMR4562 x P1(NR9915) |
SMR8365 | SMR4562 dnaE915 zae-502::Tn10 | SMR4562 x P1(NR9915) |
SMR8913 | SMR5400 polBΔ1::ΩSm-Sp | SMR5400 x P1(SMR3661) |
SMR8949 | SMR4562 dinB10 polBΔ1::ΩSm-Sp [F'dinB10] | SMR5830 x P1(SMR3661) |
SMR8950 | SMR4562 dinB10 leu::Tn10 ara+ [F'dinB10] | SMR8949 x P1(SMR6263) |
SMR8951 | SMR4562dinB10 [F'dinB10] | SMR8950 x P1(4562) |
SMR9023 | SMR4562 polA6 Tn10 | SMR4562 x P1(CM5407) |
SMR9024 | SMR4562 polA+ Tn10 | SMR4562 x P1(CM5407) |
SMR10308 | SMR4562 [F' lafU2::FRTcatFRT dinBo21(oc)] | [38] |
ZK2146 | leu::Tn10 | S.E. Finkel |
aCGSC, E. coli Genetic Stock Center, Yale University.