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. 2010 May 27;6(5):e1000973. doi: 10.1371/journal.pgen.1000973

Figure 3. Overexpression of RAD51 increases the kinetics and efficiency of BIR.

Figure 3

(A) Southern blot analysis of the kinetics of repair product in wild type and pPGK::RAD51 cycling cells as indicated in Figure 1A. Lane S contains DNA from a colony where BIR occurred. (B) Kinetics of repair are shown for PCR of BIR induced in cycling wild type (WT) and pPGK::RAD51 cells. Data are the mean ±data range. (C) Efficiency of BIR in cells as measured by viability following a DSB in a BIR assay with increased homology (2,977 bp homology). Data from Figure 2A and Figure 3D (1,157 bp homology strain) are shown for comparison. Data are the mean ±s.e.m. Values marked with asterics are statistically significant (*represents p < 0.05, ** represents p < 0.01 compared to wild type). (D) Efficiency of BIR in strains graphed in Figure 2 also carrying either pPGK::RAD51 or pADH::RAD51 as measured by viability following a DSB. Data are the mean ±s.e.m. Values marked with asterics or number sign are statistically significant (*represents p < 0.05, ** represents p < 0.01 compared to wild type. # represents p < 0.05 to the corresponding single mutant). (E) Efficiency of GC in WT and pPGK::RAD51 as measured by viability following a DSB.