Figure 5. Characterization of Can^R HPH^S sgs1Δ exo1Δ colonies in the BIR strain by PFGE.

(A) Ethidium bromide-stained agarose gel PFGE gel of sgs1Δ exo1Δ colonies that have repaired the DSB. Included are the ladder (L), starting strain prior to DSB induction (ST), Can^S HPH^S colony that has repaired by BIR (B), and twelve Can^R HPH^S colonies (1–12). Arrows indicate additional uncharacterized chromosomal fragments. (B) Southern blot analysis of (5A) by hybridization with a probe for MCH2 that normally lies 6 kb from the telomere on Ch XI (See Figure 1). (C) The blot was stripped and Southern blot analysis was performed by hybridization with a probe for CAN1 that normally lies 33 kb from the telomere on Ch V and is 1 kb proximal to the HO cut site (See Figure 1). (D) The blot was stripped and Southern blot analysis was performed by hybridization with a probe for NPR2 that normally lies 36 kb from the telomere on Ch V and is 4 kb proximal to the HO cut site (See Figure 1). (E) Southern blot analysis was performed on (5D) by hybridization with a probe for PRB1 that normally lies 40 kb from the telomere on Ch V and is 8 kb proximal to the HO cut site (See Figure 1).