Skip to main content
. 2010 May 27;6(5):e1000928. doi: 10.1371/journal.ppat.1000928

Figure 2. AICDA impacts HBV replication by hyperediting the genome.

Figure 2

A) Macroscopic impact of AICDA and A3G on HBsAg secretion into the culture supernatant following transfection of the infectious molecular clone, pCayw. B) 3DPCR recovered A3G- and AICDA-edited HBV genomes down to 86.6°C and 85.2°C respectively. pv: empty plasmid vector and HBV alone. Asterisks refer to the samples cloned and sequenced. C) Mutation matrices for hyperedited X gene sequences derived from cloned 88.7°C 3DPCR products. n indicates the number of bases sequenced. D) Bulk dinucleotide context of HBV X region minus strand DNA by eight human cytidine deaminases. E) Clonal analysis of editing for individual edited sequences. The number of TpC+CpC vs. GpC+ApC targets edited per sequence are computed and represented on the y and x axes respectively. As the A3C and A3G genes were strongly up regulated (Figure 1) they have been separated from the others. Clonal analysis using TpC vs. CpC allows clear isolation of A3G from other A3 enzymes.