Figure 5. A3 editing of the precore and HBsAg coding regions.

A) Site specific frequency analysis of A3G editing in the precore region from a tissue culture transfection experiment and from patient #326 recovered by 3DPCR at the restrictive temperature of 85.5°C. B) A selection of edited precore sequences in vivo. C) Correlation of G→A and C→T edited sites observed from the 95°C amplifications of samples #203, #318, #326 and #372 with those described from other studies with respect to the RNA stem loop structure implicit to DNA replication. D) Secondary structure of the common “a” determinant of HBsAg. The frequent G145R substitution and the G145R variant are noted along with the corresponding codon changes. E) A selection of A3 edited S region sequences bearing mutations in the G145 codon (standard PCR (95°C)). Such sequences represent <10% of the total.