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. 2010 May 27;6(5):e1000920. doi: 10.1371/journal.ppat.1000920

Figure 6. Satellite-associated Tg-satRNA localized to heterochromatin in Toxoplasma.

Figure 6

New cloned Tg-satRNA species were assigned to repetitive satellite elements Sat350 and Sat529a, which are embedded in heterochromatin domains. Tg-satRNAs with perfect matches were plotted to satellite elements Sat350 (A) and Sat529a (B). The short thin lines below the long bars represent small RNAs derived from the sense strands with the reads indicated at the left. (C) Analyses of selected modified histones ratio at satellite Sat350 and Sat529a by quantitative chromatin immunoprecipitation PCR. Ratios of DNA precipitated with target modifications over DNA precipitated with core histone H3 were used to calculate relative precipitated fold enrichment shown on the y-axis. Experiments were triplicated and the data sets are concordant. Relative intensity is shown with standard deviations.