Fig. 6.
Rat HO-1 mRNA is not expressed in dorsal skin. The dorsal skin of sickle mice was examined for the presence of rat hmox-1 mRNA by qRT-PCR RFLP analysis. The rat hmox-1 ApaI fragments at 92 and 120 bp were absent in the dorsal skin samples. The mouse product at 212 bp was seen in the skin of all mice. Rat hepatocytes were analyzed by qRT-PCR RFLP as controls and show the rat hmox-1 fragments at 92 and 120 bp but not the mouse product at 212 bp. The lane marked L designates DNA ladder