Supplementary Fig. 8.

Sequence comparison of representative ZAS proteins. Sco RsrA, Streptomyces coelicolor RsrA; Mtb RshA, Mycobacterium tuberculosis RshA; bstylac,Bacillus subtilisYlac σ factor; MSM, anti-σ factor from Mycobacterium smegmatis str. MC;²Rsp ChrR, Rhodobacter sphaeroides ChrR; Mtb RV1222, Mycobacterium tuberculosis anti-σ^E, Mycobacterium abscessus hypothetical protein MAB_2164; Mtb RslA, Mycobacterium tuberculosis RslA. The asterisk on the top of sequence alignment represents probable residues involved in zinc binding. The residues shown against a red background are involved in Zn²⁺ coordination (either cysteine or histidine). The conserved zinc binding motif residues are shaded yellow. The sequences of XX dipeptide in conserved CXXC motif in anti-σ factors are shown in pink. The secondary structural elements shown are based on the crystal structure of RslA (PDB ID 3HUG).