Fig. 3.

Pb²⁺ inhibits TRPM2 and TRPM3 channels. Data from multiwell [Ca²⁺]_i measurement from T-rex HEK 293 cells. (A) Mean response from three independent experiments for change in [Ca²⁺]_i in response to Pb²⁺ (10 μM), and H₂O₂ (1 mM) in cells with TRPM2 over-expression. (B) Example time-series graph for [Ca²⁺]_i in response to H₂O₂ (1 mM) in cells with TRPM2 over-expression incubated for 15 min in SBS with or without Pb²⁺ (10 μM). The data points represent the mean ± SEM for three wells. (C) As of (B) but showing the mean data for three independent experiments. (tet+, induced TRPM2 cells; tet−, control cells.) (D) Mean response from three independent experiments for change in [Ca²⁺]_i in response to Pb²⁺ (10 μM), and pregnenolone sulphate (PregS; 10 μM) in cells with TRPM3 over-expression. (E) Example time-series graph for [Ca²⁺]_i in response to PregS (10 μM) in cells with TRPM3 over-expression incubated for 15 min in SBS with or without Pb²⁺ (10 μM). The data points represent the mean ± SEM for three wells. (F) As of (E) but showing the mean data for three independent experiments. (tet+, induced TRPM3 cells; tet−, control cells.)