Fig. 9.

Expression of Nanog (a), Oct4 (b), Sox2 (c) and c-Jun (d) from oocytes injected with either ES or retinoic acid differentiated ES (ESRA) nuclei (see key). (e) Transcript values for Nanog, Oct4 and Sox2 in wholes ESRA cells as compared to whole ES cells prior to permeabilization. This illustrates the typical differences between ES and 4 day retinoic acid-treated ESRA cells. Error bars represent standard deviation of mean biological replicates (n is indicated in brackets for each cell-type in the key). Δ indicates one or more replicate discarded due to GV mis-targetting for that sample group. P < 0.5, unless marked ∗0.1 > P > 0.05 or ∗∗P > 0.1.