Table 2.
Fragment | Direction | Sequence (5′–3′)a | Amplicon (kb) |
---|---|---|---|
Nanog | Forward | CTCCTAATACGACTCACTATAGGGTGCCTCTTCAAGGCAGCCCTGATTCT | 2 |
Reverse | GTAGAATTAACCCTCACTAAAGGGTTGTGGGGTGCTAAAATGCGCATGGC | ||
Oct4 | Forward | AGGATAATACGACTCACTATAGGGAGCTAGAACAGTTTGCCAAGCTGCTG | 1.4 |
Reverse | CTGGTGAATTAACCCTCACTAAAGGGAGAGCCCAGAGCAGTGACGGGAAC | ||
Sox2 | Forward | GCCCGCTAATACGACTCACTATAGGGATGATGGAGACGGAGCTGAAGCC | 2.1 |
Reverse | TGTCAATTAACCCTCACTAAAGGGATCTCAAACTGTGCATAATGGAGTAA | ||
G3PDH | Forward | CAGCTCTAATACGACTCACTATAGGGATTTGGCCGTATTGGGCGCCTGG | 1.4 |
Reverse | CTTGAATTAACCCTCACTAAAGGGTTTCTTACTCCTTGGAGGCCATGTAG | ||
c-Jun | Forward | CGCTAATACGACTCACTATAGGGATGACTGCAAAGATGGAAACGACCTTC | 1.1 |
Reverse | TTGCAATTAACCCTCACTAAAGGGTTCTCAAAACGTTTGCAACTGCTGCG | ||
VegT | Forward | GTGATATAATACGACTCACTATAGGGAATGAGAAACTGCTGTCGGGAATG | 1.4 |
Reverse | GTGGATAATTAACCCTCACTAAAGGGTTACCAACAGCTGTAGGGGAAGAG |
T7 and T3 RNA promoter sequences are shown in bold. T7 is used on the forward primer for synthesis of in vitro RNA that mimics a portion of the corresponding mouse primary transcript.