Fig. 2.

Effect of CDCA on transcription of steroid-metabolising enzymes. (A) Abundance of mRNAs of rat 5β-reductase and Cyp7a1 but not 3αHSD was suppressed in H4iiE cells by CDCA (100 μM), n = 5. (B) Activity of the promoter of human 5β-reductase was reduced by CDCA (50 μM). Data are fold induction of luciferase activity relative to control plasmid. (n = 3 triplicates), mean ± SEM; ^∗p <0.05, ^∗∗∗p <0.001 vs. vehicle.