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. 2010 Jun;176(6):2722–2731. doi: 10.2353/ajpath.2010.090974

Figure 4.

Figure 4

In vitro growth of endometrial glands requires PI3K/Akt signaling. A: Western blot analysis showing phosphorylated ERK and Akt and cyclinD1 expression in endometrial glands stimulated 15 minutes or 16 hours with basal medium (BAS), BIE medium, or BIE medium plus 20 μmol/L LY294002 (LY). Membranes were reprobed to ensure equal protein loading. B: Quantification of BrdU-positive nuclei in glands growing in basal medium, BIE medium, or BIE medium plus 10 (LY10) or 20 μmol/L LY294002 (LY20).