Skip to main content
. 2010 Jun;176(6):2776–2784. doi: 10.2353/ajpath.2010.090809

Figure 2.

Figure 2

HIV induces early and sustained inhibition of CaMKII activation in hippocampal neurons. To measure CaMKII levels and activation via immunoblotting, primary rat hippocampal neurons were lysed at successive time points after exposure to HIV(Jago)/MDM (triangles). Mock/MDM-exposed neurons (circles) were prepared for control comparison at each time point. A: Total CaMKII was not altered in HIV/MDM-exposed neurons. B: In contrast, CaMKII activation (autophosphorylation) began declining 30 minutes after exposure to HIV/MDM and continued to decrease until 4 hours postexposure (P = 0.015). C: The ratio of active to total CaMKII also decreased beginning at 30 minutes postexposure and continuing to the 4-hour postexposure time point (P = 0.016).