Figure 5.

CWD PrP^RES in tissues of p.o. inoculated and horizontally infected Tg[CerPrP] mice. All tissues were obtained from either a terminally ill, PLP-perfused, p.o. inoculated mouse sacrificed at 350 dpi (A–C) or from a terminally ill, PLP-perfused, noninoculated mouse that cohabitated with an i.c. inoculated mice that was sacrificed because of terminal neurological disease (D–F). All tissues were immunostained using the IHC protocol detailed in Materials and Methods. Each panel demonstrates PrP^RES immunoreactivity (brown) in selected tissues. A: PrP^RES in the cervical spinal cord, including heavy deposition surrounding the central canal. B: PrP^RES in the granular layer of the cerebellum. C: PrP^RES in the cells of the Peyer’s patch which, based on size and cytomorphologic characteristics, are probably macrophages and/or dendritic cells. D: PrP^RES in the brainstem. E: PrP^RES in the hippocampus (hilus fasciae dentatae). F: PrP^RES in the pancreatic islets. Scale bars: 25 μm (A); 75 μm (B); 200 μm (C); 25 μm (D); 60 μm (E); 50 μm (F).