Table 3.
Association of Immunocytochemical Staining for Cytoplasmic FANCD2 with Other Tumor Variables
| Tumor variable* | FANCD2 negative no†(%) | FANCD2 positive no† (%) | Statistical significance‡
|
|
|---|---|---|---|---|
| Uncorrected | Corrected | |||
| LN− | 36 (42.9) | 80 (56.3) | 0.055 | 0.57 |
| LN+ | 48 (57.1) | 62 (43.7) | ||
| Tumor <5 cm | 78 (69) | 147 (79) | 0.055 | 0.57 |
| Tumor >5 cm | 35 (31) | 39 (21) | ||
| GradeI+II | 65 (62.5) | 140 (80.9) | 0.0011 | 0.016 |
| Grade III | 39 (37.5) | 33 (19.1) | ||
| OPN− | 11 (9.6) | 86 (46) | <0.0001 | <0.0001 |
| OPN+ | 103 (90.4) | 101 (54) | ||
| c-erbB-2 | 83 (72.2) | 155 (80.3) | 0.122 | 0.86 |
| c-erbB-2+ | 32 (27.8) | 38 (19.7) | ||
| c-erbB-3− | 51 (46.4) | 69 (36.1) | 0.088 | 0.75 |
| c-erbB-3+ | 59 (53.6) | 122 (63.9) | ||
| S100A4− | 42 (35.6) | 145 (74.7) | <0.0001 | <0.0001 |
| S100A4+ | 76 (64.4) | 49 (25.3) | ||
| S100P− | 33 (32) | 94 (55.6) | 0.0002 | 0.0025 |
| S100P+ | 70 (68) | 75 (44.4) | ||
| ERα− | 59 (52.2) | 83 (43) | 0.124 | 0.86 |
| ERα+ | 54 (47.8) | 110 (57) | ||
| PgR− | 76 (67.3) | 105 (56.8) | 0.087 | 0.74 |
| PgR+ | 37 (32.7) | 80 (43.2) | ||
| p53− | 69 (59) | 120 (61.5) | 0.720 | 1.00 |
| p53+ | 48 (41) | 75 (38.5) | ||
| Cathepsin D− | 20 (21.5) | 25 (16.4) | 0.39 | 1.00 |
| Cathepsin D+ | 73 (78.5) | 127 (83.6) | ||
| pS2− | 70 (61.4) | 113 (58.2) | 0.088 | 0.75 |
| pS2+ | 44 (38.6) | 81 (41.8) | ||
| AGR2− | 12 (11.2) | 89 (46.4) | <0.0001 | <0.0001 |
| AGR2+ | 95 (88.8) | 103 (53.6) | ||
| Ki67− | 34 (47.9) | 45 (36.3) | 0.13 | 0.88 |
| Ki67+ | 37 (52.1) | 79 (63.7) | ||
LN, lymph node with tumor (+) or without tumor deposits (−); tumor size >5 cm (+),<5 cm in diameter (−); grade, histological grade III versus histological grades I and II; the presence (+) or absence (−) of immunocytochemical staining for osteopontin (OPN), c-erbB-2, c-erbB-3, S100A4, S100P, progesterone receptor (PgR), p53, pS2 using a 5% cut-off and the presence (+) or absence (−) of staining for cathepsin D, AGR2 and nuclear Ki67 using a 1% cut-off (Materials and Methods).
Number of patients with carcinomas either classified as staining (positive) or not staining (negative) for cytoplasmic FANCD2 using a 1% cut-off between the positive and the negative groups. Brackets show the percentage of patients.
Probability, P from Fisher’s Exact test (two sided values) either uncorrected or corrected using the Holm-Bonferroni adjustment 1 − (1 − P)n, where n = 15 (Materials and Methods).