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Shedding of TMPRSS2 to the extracellular milieu by LNCaP cells is very limited. LNCaP cells were grown in serum-free media for 24 hours, and then the conditioned medium and cell lysates were harvested. The conditioned medium was concentrated up to 100 fold. The cell lysates (C) and the conditioned medium (M) were analyzed for total matriptase (Total MTP) using the matriptase mAb M32 and TMPRSS2 using the mAb AL20.
