Figure 6.

TMPRSS2 is present in multiple complexes in human semen. A: Equal amount of human semen were mixed with SDS sample buffer in the absence (NR) or presence (R) of reducing agents and incubated either at room temperature (NB) or 95°C (B) for 5 minutes, followed by SDS-PAGE and Western blot analysis using TMPRSS2 mAb AL20. B: Human semen was resolved by diagonal gel electrophoresis under nonreducing and boiled conditions in the first dimension and under reducing and boiled conditions in the second dimension. Immunoblot analysis of the second dimensional gel was performed with the TMPRSS2 mAb AL20.