Table 1.
TaqMan results for the 86 strains of mycoplasmas tested in this study.
| Species | Straina | Origin | Isolated | Host | TaqMan |
|
|---|---|---|---|---|---|---|
| FTb | MGBb | |||||
| M. mycoides subsp. mycoides SC | PG1 | 1931 | Cattle/type strain | + | + | |
| 2059 | Spain | 1984 | Cattle/lung | + | + | |
| B773/125 | Portugal | 1991 | Cattle/semen | + | + | |
| C305 | Portugal | 1993 | Goat/lung | + | + | |
| O326 | Portugal | 1993 | Sheep/milk | + | + | |
| PO 1967 | France | 1967 | Cattle/lung | + | + | |
| PO 2 | France | 1980 | Cattle/lung | + | + | |
| 2022 | France | 1984 | Cattle/lung | + | + | |
| L2 | Italy | 1993 | Cattle/lung | + | + | |
| 402 | Italy | 1990 | Cattle/lung | + | + | |
| 6479 | Italy | 1992 | Cattle/lung | + | + | |
| Afadé | Cameroon | 1968 | Cattle/lung | + | + | |
| 87137-9 | Burkina Faso | 1987 | Cattle | + | + | |
| Filfili | Senegal | Pre-1988 | Cattle | + | + | |
| Fatick | Senegal | 1968 | Cattle | + | + | |
| B17 | Chad | 1967 | Zebu | + | + | |
| 9050-529/1 | Ivory Coast | 1990 | Cattle | + | + | |
| 91130 | Central African Republic | 1991 | Cattle | + | + | |
| 94111 | Rwanda | 1994 | Cattle | + | + | |
| 95014 | Tanzania | 1995 | Cattle | + | + | |
| T1/44 | Tanzania | 1952 | Cattle/vaccine strain | + | + | |
| T1lppQ−MT1 | DIVA mutant | 2008 | Transposon-based random mutagenesis | − | + | |
| T1lppQ−RSP1 | Recombinant mutant | 2008 | Transposon-based random mutagenesis | + | + | |
| T1/Sr50 | Tanzania | 1952 | Cattle/vaccine strain | + | + | |
| KH3J | Sudan | 1940 | Cattle/vaccine strain | + | + | |
| Gladysdale | Australia | Pre-1964 | Cattle | + | + | |
| DVZ | Australia | 1965 | Cattle | + | + | |
| R575 | Australia | 1965–1968 | Cattle | + | + | |
| V5 | Australia | 1936 | Cattle/vaccine strain | + | + | |
| Mycoplasma leachii | PG50 | Australia | 1963 | Cattle/joint/reference strain | + | − |
| B5415 | Portugal | Cattle | + | − | ||
| CP291 | Portugal | Goat/lung | + | − | ||
| PAD3186 | India | 1993 | Goat/milk | + | − | |
| FRD424 | India | 1993 | Goat/milk | + | − | |
| Calf 1 | Nigeria | Cattle/blood | + | − | ||
| D318b | Germany | Cattle/semen | + | − | ||
| C2306 | Portugal | Cattle | + | − | ||
| D424 | Germany | Cattle/preputium | + | − | ||
| QR1/92 | Australia | + | − | |||
| 4055 | France | Cattle/lung | + | − | ||
| B144P | USA | 1956 | Cattle/joint | + | − | |
| M. mycoides subsp. capri | PG3 | Turkey | 1950 | Goat/type strain | − | − |
| N108 | Nigeria | 1977 | Goat | − | − | |
| Capri L | France | 1975 | Goat | − | − | |
| 9139/11-91 | Turkey | 1991 | − | − | ||
| WK354/80 | Switzerland | 1980 | Goat | − | − | |
| 213 | India | 1984 | Goat | − | − | |
| Y-goat | Australia | 1956 | Goat/type strain (serovar MmmLC) | − | − | |
| 152/93 | Grand Canary | 1993 | Goat | − | − | |
| LC8065 | France | Goat | − | − | ||
| D2482/91 | Switzerland | 1991 | Goat | − | − | |
| 950010 | France | 1995 | Goat | − | − | |
| D2083/91 | Switzerland | 1991 | Goat | − | − | |
| CP271 | Portugal | 1991 | Goat | − | − | |
| D2503 | Switzerland | Goat | − | − | ||
| M. capricolum subsp. capricolum | California kid | USA | 1955 | Goat/type strain | − | − |
| 173/87 | Greece | 1987 | Sheep | − | − | |
| 6443.90 | France | 1990 | Goat | − | − | |
| M. capricolum subsp. capripneumoniae | F38 | Kenya | 1976 | Goat/type strain | − | − |
| 9081-487p | Oman | 1990 | Goat | − | − | |
| Gabès | Tunisia | 1981 | Goat | − | − | |
| M. bovis | PG45 | USA | 1962 | Cattle/milk/type strain | − | − |
| ML1 | France | Pre-1999 | Rabbit/lung | − | − | |
| 120/81 | Germany | 1980–1990 | Cattle/milk | − | − | |
| 221/89 | Germany | 1980–1990 | Cattle/milk | − | − | |
| 86p | Belgium | 1990–2000 | Cattle/milk | − | − | |
| 39G | Belgium | 1990–2000 | Cattle/bronchoalveolar lavage fluid | − | − | |
| 0435 | Belgium | 1990–2000 | Cattle/bronchoalveolar lavage fluid | − | − | |
| 9585 | Belgium | 1990–2000 | Cattle/bronchoalveolar lavage fluid | − | − | |
| 2610 | UK | 1990–2000 | Cattle/joint fluid | − | − | |
| 2138 | UK | 1990–2000 | Cattle/milk | − | − | |
| 2960 | UK | 1990–2000 | Cattle/lung | − | − | |
| M. bovirhinis | PG43 | 1967 | Cattle/respiratory tract/type strain | − | − | |
| O/D1467 | Switzerland | 2007 | Cattle/bronchus | − | − | |
| IMD1656 | Switzerland | 2008 | Cattle/bronchus | − | − | |
| M. bovigenitalium | PG11 | United Kingdom | Cattle/type strain | − | − | |
| 2D | Australia | 1974 | Sheep (former serogroup 11 strain) | − | − | |
| M. bovoculi | RF20391 | Cattle | − | − | ||
| M. agalactiae | PG2 | Spain | 1973 | Goat/type strain | − | − |
| 3990 | France | − | − | |||
| 5725 | France | 1990 | Sheep | − | − | |
| M. putrefaciens | KS1 | USA | Goat/type strain | − | − | |
| M. ovipneumoniae | Y98 | Sheep/respiratory tract/type strain | − | − | ||
| M. conjunctivae | HRC/581 | USA | 1972 | Sheep/type strain | − | − |
| M. hyopneumoniae | J | 1965 | Swine/type strain | − | − | |
| M. arginini | G230 | Mouse/brain/type strain | − | − | ||
Collections: the strains were obtained from the Australian Animal Health Laboratory (AAHL), Geelong, Victoria, Australia; Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Lyon, France; BGVV, Jena, Germany; CIRAD-EMVT, Montpellier, France; Faculdad de Veterinaria, Universidad de Las Palmas, Spain; INRA, Villenave d'Ornon, France; Institute of Veterinary Bacteriology, University of Bern, Switzerland; Laboratório Nacional de Investigação Veterinária, Lisboa, Portugal; Laboratoire de Pathologie Bovine, Lyon, France; National Collection of Type Cultures (NCTC), PHLS, London, UK; National Veterinary Institute, Uppsala, Sweden; and Faculty of Veterinary Medicine, University of Liège, Belgium.
As determined in this study by real-time PCR of approximately 10 ng of genomic DNA. FT, TaqMan assay with the lppQ-based FAM probe (Bischof et al., 2006); MGB, TaqMan assay with the lppQ-based minor groove binding probe (Vilei et al., 2007).