Table 3.
Comparison between TaqMan MGB assay-calculated mycoplasmal loads in artificially contaminated bronchoalveolar lavage fluids and inoculated CFU counts.
| Inoculateda (CFUreaction) | C305 |
O326 |
2022 |
|||
|---|---|---|---|---|---|---|
| Loadreactionb | Δ(%)c | Loadreactionb | Δ(%)c | Loadreactionb | Δ(%)c | |
| 7 × 106 | 5.52 × 106 (± 3.72 × 105) | − 21.08 | 4.93 × 106 (± 3.76 × 105) | − 29.61 | 5.23 × 106 (± 8.94 × 105) | − 25.23 |
| 7 × 105 | 5.94 × 105 (± 3.47 × 104) | − 15.16 | 5.74 × 105 (± 2.58 × 104) | − 18.07 | 5.57 × 105 (± 1.03 × 105) | − 20.38 |
| 7 × 104 | 6.19 × 104 (± 5280) | − 11.64 | 6.41 × 104 (± 5180) | − 8.50 | 6.51 × 104 (± 3801) | − 7.03 |
| 7 × 103 | 6524 (± 1260) | − 6.80 | 6974 (± 250.7) | − 0.37 | 6864 (± 339.2) | − 1.94 |
a
CFU counts present in 2.5 μl of bronchoalveolar lavage fluids contaminated artificially with 10-fold diluted suspensions of the three strains C305, O326 and 2022.
b
Mycoplasmal loads obtained by applying the formula loadreaction = 1.3494 × 1012 × e− 0.63539 × Ct generated by the linear regression of the standard curve from the M. mycoides subsp. mycoides SC-specific TaqMan MGB assay. Four data sets for each strain and each dilution were used.
c
Percent difference between calculated mycoplasma loads and inoculated CFU counts.