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. Author manuscript; available in PMC: 2010 May 27.
Published in final edited form as: Mol Ther. 2007 Mar 13;15(5):954–961. doi: 10.1038/sj.mt.6300086

Figure 3. Analysis of GFP-specific CTL responses.

Figure 3

(a) Stimulated lymphocytes (effector cells) were harvested and viable effector cells were mixed with GFP-expressing syngeneic fibroblasts at different effector:target ratios (50:1 to 3:1) and incubated for 5 h. GFP-tolerant mice grafted with GFP+ keratinocyte were used as controls. Specific lysis of target cells was determined in triplicates using LZ-expressing syngeneic fibroblasts as control targets. (b) Mice were transduced in vivo (n = 4) with INV-GFP or ex vivo (n=6) with LZRS-GFP, and at 7 weeks posttransduction when GFP expression was lost in all mice, they were challenged with transplantation of GFP expressing keratinocytes. Mice were monitored weekly and GFP-expression in the grafted area was scored. A ≥90% decrease in surface GFP was taken to indicate graft loss.