Fig. 6.

Measurement of VSG intensity on the cell surface of trypanosomes in ERAP32 RNAi cells. (A) Fluorescence intensity comparison for VSG between induced and uninduced cells without permeabilization following 1 day induction of ERAP32 RNAi. (A) VSG intensity was measured on individual cells by fluorescence microscopy. The VSG-associated fluorescent intensity of each cell was measured under identical exposure parameters. Data are presented individually and also as mean ± SD of 50 cells for each sample in a scatter graph. ∗∗P < 0.01. (B) Two representative images from uninduced and induced cells stained for VSG.