Fig. 7.

Effect of silencing ERAP32 or ERAP18 on VSG exocytosis. (A) VSG export was monitored by pulse-chase labeling with [³⁵S]-labeled methionine/cysteine, followed by hypotonic lysis to release surface VSG via action of endogenous GPI-PLC. Data represent the kinetics of newly synthesized VSG transported from the endomembrane system to the cell surface. The experiment was performed as described in Allen et al., 2003. Data were from two independent experiments and error bars indicate the standard error. (B) Metabolic labeling of newly synthesized VSG. Newly synthesized VSG within 10 min were labeled with [³⁵S]-labeled methionine/cysteine and detected by autoradiography. The experiment was done twice with identical results. Lanes presented are from the same gel, and manipulated solely for presentation purposes.