Figure 2.

Western blot analysis of soluble N-ethyl maleimide-sensitive factor attachment protein receptor (SNARE) complex proteins in megakaryocytes. Expression of SNARE complex proteins SNAP-23, Syntaxin 4, and vesicle-associated membrane protein (VAMP) was determined by Western blot analysis in MEG-01 cells, in the undifferentiated (−phorbol myristate acetate [PMA]), as well as differentiated (+PMA) state, and in primary human megakaryocytes (MK). Human brain (HB) lysate was used as a positive control, detection of glyceraldehyde phosphate dehydrogenase (GAPDH) was performed as loading control.