Table 1.
Pre-treatment | Z. japonica |
CA1P (nmol mg−1 Chl) | |
Dark | 5.97±1.76 |
Light | 0.53±0.39 |
Samples were taken from well-watered plants after a period of 12 h in darkness (Dark) or 4 h after the beginning of the subsequent photoperiod (Light). The inhibitor was extracted with trifluoroacetic acid and measured after HPLC separation. Extracts were spiked with tracer quantities of [14C]-CA1P to allow correction for losses in processing the samples (average 56.4%). The retention time in the chromatographic system was the same as authentic CA1P and consistent with the radioactive spike. Values are means of four biological replicates ±standard errors.