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. 2010 Apr 2;61(9):2355–2366. doi: 10.1093/jxb/erq071

Table 1.

Quantity of CA1P (nmol mg−1 chlorophyll) in light- and dark-adapted leaves of Zoysia japonica

Pre-treatment Z. japonica
CA1P (nmol mg−1 Chl)
Dark 5.97±1.76
Light 0.53±0.39

Samples were taken from well-watered plants after a period of 12 h in darkness (Dark) or 4 h after the beginning of the subsequent photoperiod (Light). The inhibitor was extracted with trifluoroacetic acid and measured after HPLC separation. Extracts were spiked with tracer quantities of [14C]-CA1P to allow correction for losses in processing the samples (average 56.4%). The retention time in the chromatographic system was the same as authentic CA1P and consistent with the radioactive spike. Values are means of four biological replicates ±standard errors.