Fig. 5.

Levels of GFP protein in the promoter:gfp transgenic rice plants. Protein extracts were prepared from the leaf and root tissues at 30 DAG and from flowers just before pollination. Three independent promoter:gfp transgenic plants and non-transgenic (NT) plants were analysed. One line of each of the transgenic plants harbouring the OsCc1 (Jang et al., 2002), Act1 (McElroy et al., 1990), and ZmUbi1 (Cornejo et al., 1993) gene promoters were included as constitutive controls. Ten μg of total soluble proteins were separated on a SDS-polyacrylamide gel, transferred to a membrane, and then immunoblotted with an anti-GFP antibody. The amount of GFP protein in promoter:gfp transgenic plants were calculated by comparing band intensities with those of known amounts of recombinant GFP protein used as positive controls (PC).