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. 2010 Apr 27;61(9):2499–2506. doi: 10.1093/jxb/erq085

Fig. 3.

Fig. 3.

Enhanced seed germination of aged CA plants under single or combined abiotic stress conditions. Twenty-five NT and CA seeds were germinated and the germination rates were recorded in Murashige-Skoog (MS) media with various environmental stress conditions for 4 weeks at 25 °C except low temperature (L) treatment at 15 °C. (A) Photographs of 1-week-old aged NT and CA seeds grown in MS medium (MS) or in MS medium treated with osmotic stress (O, MS media added with 4% PEG), salt (S, MS media added with 100 mM NaCl), or low temperature (L, grown at 15 °C). (B) Photographs of 1-week-old aged NT and CA seeds grown in MS medium under combined stress conditions, such as osmotic stress and salt (OS), low temperature and osmotic (LD), low temperature and salt (LS), and triple combined stress (LOS). (C) Germination rates of NT and CA seeds in MS media treated with environmental stressors. (a) Seed germination rates of new NT and CA seeds in an MS medium for 4 weeks. Seed germination rates were observed for 4 weeks for aged NT and CA seeds in an MS medium (b), and in an MS medium treated with osmotic stress (c), salt (d), low temperature (e), osmotic stress and salt (f), low temperature and osmotic stress (g), low temperature and salt (h), or low temperature, osmotic stress, and salt combined stresses (i). Data are presented as mean ±SD of three independent assays. (This figure is available in colour at JXB online.)