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. 2010 May 17;120(6):2016–2029. doi: 10.1172/JCI39896

Figure 8. Shh is a critical mediator of the angiogenic effects of EPO in vitro.

Figure 8

The expression levels of VEGF protein (A) and Ang-1 mRNA (B). Cardiomyocytes were treated with EPO for 48 hours. Cyclopamine (5 μM) was added before EPO treatment. Quantification of the bands is shown (n = 4). *P < 0.05. (C) HUVEC proliferation assay. HUVECs were treated with cardiomyocyte-conditioned medium. Cyclopamine was added before EPO treatment. n = 5 per condition. (D) Detection of apoptosis using Cy3-labeled annexin V. Quantitative analysis is shown (n = 6).