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. 2010 Feb 1;9(6):1085–1099. doi: 10.1074/mcp.M900381-MCP200

Fig. 7.

Fig. 7.

A, relative quantities of the secreted MMP-13-TIMP proteolytic system components, MMP-13 and TIMP-1, -2, and -4, determined by cytokine arrays. MMP-13 secretion was found to be elevated by 450, 585, and 300% by 48-h Hx, 72-h Hx, and Reox, respectively, whereas TIMP levels wersuppressed under both Hx and Reox. B, relative levels of secreted ANG and VEGF as determined by cytokine array. Both were significantly elevated under Hx, but ANG dropped precipitously to 65% of control under Reox, whereas secreted VEGF levels increased further. Statistical significance was accepted at p < 0.05 (*) and p < 0.01 (**). C, relative quantities of the secreted MMPs in MDA-MB-231, A549, and H1299 cells determined by cytokine arrays. MMP-3, -9, and -13 secretion was found to be elevated under 72-h Hx. D, relative levels of secreted chemokines related to recruitment of immune cells, G-CSF, GM-CSF, CXCL-16, and stroma-derived factor-1 (SDF-1beta), as determined by cytokine array. Secretion of these chemokines was significantly elevated under Hx. 48N, 48-h normoxia; 48H, 48-h hypoxia; 48H-24R, 48-h hypoxia followed by 24-h normoxia.