. 2010 Feb 1;9(6):1100–1117. doi: 10.1074/mcp.M900398-MCP200

Table II. Predicted secretion pathways of proteins identified in conditioned media from 23 cancer cell lines.

Cell line	No. of identified proteins				Percentage of predicted secreted proteins
Cell line	Classical secretion^a	Nonclassical secretion^b	Membrane protein^c	Others^d	Percentage of predicted secreted proteins
NPC-TW02	255	235	14	337	59.9
NPC-TW04	284	304	16	463	56.6
NPC-BM1	292	324	17	496	56.1
Colo205	295	430	34	563	57.4
SW480	213	250	17	377	56.0
SW620	229	468	16	727	49.5
SK-Hep-1	269	293	16	376	60.6
HepG2	267	206	12	290	62.6
Hep-3B	383	573	39	824	54.7
OEC-M1	333	422	29	559	58.4
SCC-4	209	487	27	783	48.0
U1	280	305	18	468	56.3
U4	364	687	44	1,047	51.1
MCF-7	219	390	23	686	48.0
MDA-MB-435S	253	589	17	921	48.3
C-33A	285	384	25	590	54.0
HeLa	243	350	9	621	49.2
CL_1–0	174	369	14	539	50.8
CL_1–5	364	621	41	804	56.1
PANC-1	224	319	19	473	54.3
MIA-PaCa-2	266	522	20	801	50.2
A-431	299	476	23	660	54.7
Jurkat	195	796	21	1,269	44.4
All 23 cell lines	998	1,438	121	2,027	55.8

^a Proteins predicted by the SignalP program to be secreted via the classical secretion pathway (SignalP probability ≥0.90).

^b Proteins predicted to be secreted by the nonclassical secretion pathway using SignalP and SecretomeP (SignalP probability <0.90 and SecretomeP score ≥0.50).

^c Proteins predicted by the TMHMM to form integral membrane proteins that were not predicted to be secreted via the classical or nonclassical secretion pathways.

^d Proteins that could not be classified as classical secreted, nonclassical secreted, or integral membrane proteins.